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Last Updated: Sep 11, 2026
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ASCP ASCP-MLT Practice Q&A's
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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Microbiology | 15-20% | - Identification of pathogenic microorganisms - Specimen collection and culture techniques - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility |
| Blood Banking (Immunohematology) | 15-20% | - Blood group systems and antigens - Transfusion reactions and adverse events - Blood components and storage - Antibody detection and identification - Compatibility testing |
| Immunology and Serology | 5-10% | - Immune system principles and components - Infectious disease serology - Immunologic testing methodologies - Autoimmune and immunodeficiency disorders |
| Hematology | 20-25% | - Red blood cell physiology and disorders - Hemostasis and coagulation testing - White blood cell morphology and diseases - Routine and automated hematology procedures |
| Laboratory Operations | 5-10% | - Quality control and quality assurance - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance |
| Urinalysis and Other Body Fluids | 5-10% | - Physical, chemical, and microscopic analysis - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing - Urine formation and composition |
| Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Hemoglobin H is a tetramer made of four beta globin chains. Hemoglobin H occurs when there is very limited alpha chain availability used to make normal hemoglobin A. Hemoglobin H forms in those affected with alpha thalassemia major as well as in people with the combination of two-gene deletion alpha thalassemia and hemoglobin Constant Spring.
Hematology
Which of the following combination of globin chains comprise Hemoglobin H?
- A. 4 alpha chains
- B. 2 alpha, 2 gamma
- C. 4 beta chains
- D. 2 alpha, 2 beta
Correct Answer: C 🗳️
One of the key characteristics to the identification of Nocardia asteroides is its inability to hydrolyze casein, tyrosine or xanthine, as shown in this photograph. Nitrates are reduced to nitrites. Both Nocardia brasiliensis and Actinomadura madurae hydrolyze both casein and tyrosine; Streptomyces griseus hydrolyzes all three of the substrates.
Illustrated in this photograph is an agar quadrant plate containing casein (A), tyrosine (B), nitrate (C) and xanthine (D). None of the substrates have been hydrolyzed and nitrate has been reduced. The most likely identification is:
- A. Actinomadura madurae
- B. Streptomyces griseus
- C. Nocardia brasiliensis
- D. Nocardia asteroides
Correct Answer: D 🗳️
Which of the following is a current title for clinical laboratory professionals with a 4 year degree?
- A. medical technologist
- B. medical laboratory scientist
- C. clinical laboratory technician
- D. medical laboratory technician
Correct Answer: B 🗳️
Troponin is potentially more specific for myocardial damage than CK-MB and stays elevated longer. It may eventually replace CK-MB as the standard marker of myocardial damage. In addition, troponin T has been known to be elevated in the setting of even mild degrees of renal failure. Troponin and CK-MB both tend to rise approximately 3 hours after a MI (hence why the correct answer is "A", since it is false); however, troponins can stay elevated up to 2 weeks as CK-MB tends to return to baseline around 36 hours.
Chemistry
Which of the following statements is FALSE regarding troponin?
- A. It stays positive much longer than CK-MB
- B. It is potentially more specific than CK-MB
- C. It rises much sooner after an MI than CK-MB
- D. Troponin T is often elevated in renal failure patients
Correct Answer: C 🗳️
1. B
2. D
3. A
4. C
Red to Brown Urine: porphobilinogen, hematuria, myoglobinuria, etc.
Green: Food colorings; Increased carotene in the diet;
Pseudomonas aeruginosa infection
Yellow: bilirubin, bile pigments
White: phosphates, other crytals
Match urine color with substance that might have been responsible:
1. Phosphates
2. Bilirubin
3. Pseudomonas
4. Porphobilinogen
- A. Blue to green
- B. Red to brown
- C. White
- D. Yellow
Correct Answer: A,B,C,D 🗳️
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